Overview

  • Product nameAnti-Hsp90 antibody [H90-10]
    See all Hsp90 primary antibodies
  • Description
    Mouse monoclonal [H90-10] to Hsp90
  • Specificityab58950 recognizes both Hsp90 alpha and Hsp90 beta
  • Tested applicationsSuitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
  • Immunogen

    Purified full length human Hsp90 beta

  • Positive control
    • Heat-shocked HeLa cell lysate.
  • General notesDilute in PBS or medium which is identical to that used in the assay system.

Properties

Applications

Our Abpromise guarantee covers the use of ab58950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
IP Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.

Cells were grown on sterile coverslips then fixed with 3.7% formaldehyde in PBS for 10 minutes and permeabilized with 0.2% Triton X-100 for 10 minutes at room temperature.

Flow Cyt Use 0.01-0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
  • Post-translational
    modifications
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heat shock 86 kDa antibody
    • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
    • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
    • Heat shock protein HSP 90 alpha antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 84 antibody
    • HSP 86 antibody
    • Hsp 90 antibody
    • HSP86 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90AB1 antibody
    • HSP90B antibody
    • HSPC1 antibody
    • HSPC2 antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Anti-Hsp90 antibody [H90-10] images

  • Overlay histogram showing HeLa cells stained with ab58950 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58950, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


  • Predicted band size : 83 kDa

    Image from Niture SK et al, J Biol Chem. 2010 Nov 19;285(47):36865-75. Epub 2010 Sep 23, Fig 6. DOI 10.1074/jbc.M110.175802.

    Western Blot analysis of the effect of siRNA inhibition of Hsp90 on INrf2. Murine hepatoma (Hepa-1) cells were transfected with control siRNA or Hsp90-specific siRNA and Hsp90 (ab58950), INrf2, and actin levels were analyzed by immunoblotting.

References for Anti-Hsp90 antibody [H90-10] (ab58950)

This product has been referenced in:
  • Shim JS  et al. Selective Inhibition of HER2-Positive Breast Cancer Cells by the HIV Protease Inhibitor Nelfinavir. J Natl Cancer Inst 104:1576-90 (2012). Human . Read more (PubMed: 23042933) »
  • Niture SK & Jaiswal AK Hsp90 interaction with INrf2(Keap1) mediates stress-induced Nrf2 activation. J Biol Chem 285:36865-75 (2010). WB ; Mouse . Read more (PubMed: 20864537) »

See all 2 Publications for this product

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