Overview

  • Product name
    Anti-Hsp90 antibody [S88]
    See all Hsp90 primary antibodies
  • Description
    Mouse monoclonal [S88] to Hsp90
  • Specificity
    The antibody recognizes a 90 kDa protein, corresponding to the apparent molecular weight of Hsp90 on SDS-PAGE immunoblots. This antibody detects both Hsp90 alpha and beta.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Full length native protein (purified) corresponding to Hsp90. Full length native protein (purified) (Achlya ambisexualis).

  • Positive control
    • Heated shocked HeLa cell lysate; Heated shocked 3T3 cell lysate; Heat shocked PC-12 cell lysate; Heat shocked CHO-K1 cell lysate; Heat shocked Rat-2 cell lysate; and recombinant Hsp90 protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.2% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1% BSA, 48% PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    S88
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IP Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 90 kDa. Lysates should be heat-shocked to detect Hsp90.
ICC/IF Use at an assay dependent concentration. PubMed: 23209418

Target

Images

  • Immunofluorescence analysis of human TIVE-L1 cells labeling HSP90 with ab1429 at 1:500 dilution. LANA was also labeled in red. Cells were fixed with 3% paraformaldehyde for 20mins, permeabilized with 0.2% Triton X-100 for 15mins and incubated in blocking buffer followed by the antibody. Slides were then incubated with the secondary antibody anti-mouse FITC. Nuclear staining with DAPI is shown in blue.

  • Anti-Hsp90 antibody [S88] (ab1429) + HeLa cell lysate

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • Overlay histogram showing HeLa cells stained with ab1429 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1429, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Aas A  et al. Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy. PLoS One 12:e0168182 (2017). WB ; Human . Read more (PubMed: 28288181) »
  • Akahane K  et al. HSP90 inhibition leads to degradation of the TYK2 kinase and apoptotic cell death in T-cell acute lymphoblastic leukemia. Leukemia 30:219-28 (2016). Read more (PubMed: 26265185) »

See all 22 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Blocking step
0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 31 2014

Vielen Dank dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

Um Ihr Problem zu lösen, möchte ich die folgenden...

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Vielen Dank für Ihre Anfrage.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Frage...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cancer cell line, HCT116)
Loading amount
100 µg
Specification
Colon cancer cell line, HCT116
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Loading amount
10 µg
Specification
HL60
Treatment
10nM Bortezomib (6&24h)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (REH, SD1 cell lines)
Loading amount
10 µg
Specification
REH, SD1 cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in PBS-
Username

Dr. Mark Holland

Verified customer

Submitted Mar 30 2007

Thank you for your rapid reply. I am very concerned that reduced conditions in western blotting did not give you bands, we have not received any other complaints about this antibody which is very popular. Could you please clarify the incubation t...

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I'm very sorry to hear you are experiencing problems with ab1429 in dot blot and in western blot. We have not tested this antibody in dot blot or in native western blotting and therefore the problem may be due to the fact that the protein in your sampl...

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Thank you for your reply, and your clarification. There are a couple of suggestions I would like to make: - I would recommend switching your blocking and primary incubation steps, i.e. blocking for 1 hour at room temperature and incubating with t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (Rat Penis)
Total protein in input
60 µg
Specification
Rat Penis
Immuno-precipitation step
Protein A/G
Username

Miss. Gwen Lagoda

Verified customer

Submitted Jun 28 2006

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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