• Product nameAnti-Hsp90 beta antibody
    See all Hsp90 beta primary antibodies
  • Description
    Rabbit polyclonal to Hsp90 beta
  • SpecificityDetects endogenous levels of total HSP90B protein.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E ).

  • Positive control
    • Extracts from Hela cells treated with TNF-a, human breast carcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab51145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.


  • FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • Post-translational
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    S-nitrosylated; negatively regulates the ATPase activity.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Anti-Hsp90 beta antibody images

  • All lanes : Anti-Hsp90 beta antibody (ab51145) at 1/500 dilution

    Lane 1 : extracts from Hela cells treated with TNF-a
    Lane 2 : extracts from Hela cells treated with TNF-a with immunizing peptide

    Lysates/proteins at 0.02 µg/ml per lane.

    Predicted band size : 83 kDa
    Observed band size : 87 kDa (why is the actual band size different from the predicted?)
  • Ab51145 at 1/50 dilution staining Human breast carcinoma, with and without blocking peptide; paraffin embedded.
  • ICC/IF image of ab51145 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51145, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Hsp90 beta antibody (ab51145)

This product has been referenced in:
  • Wang H  et al. Hsp90a forms a stable complex at the cilium neck for the interaction of signalling molecules in IGF-1 receptor signalling. J Cell Sci 128:100-8 (2015). Read more (PubMed: 25359884) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (heart tissue)
Loading amount 50 µg
Specification heart tissue
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Submitted Mar 10 2010