Overview

  • Product nameAnti-Hsp90 beta antibody
    See all Hsp90 beta primary antibodies
  • Description
    Rabbit polyclonal to Hsp90 beta
  • SpecificityDoes not cross react with Hsp90alpha
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified recombinant human Hsp90 beta

  • Positive control
    • HeLa cell lysate (20µg)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.4
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
IHC-Fr 1/100.
WB 1/1000. Predicted molecular weight: 83 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • Post-translational
    modifications
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90 alpha family class B member 1 antibody
    • Heat shock protein 90 kDa antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein 90kDa alpha family class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Anti-Hsp90 beta antibody images

  • ab80159 at 1/100 dilution staining Hsp90 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/10 dilution.

  • All lanes : Anti-Hsp90 beta antibody (ab80159)

    Lane 1 : Molecular weight ladder
    Lane 2 : Human Hsp90 beta protein at 2 µg
    Lane 3 : Human Hsp90 beta protein at 2 µg
    Lane 4 : Molecular weight ladder
    Lane 5 : Human Hsp90 alpha protein
    Lane 6 : Human Hsp90 alpha protein


    Predicted band size : 83 kDa

References for Anti-Hsp90 beta antibody (ab80159)

ab80159 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (primary cancer-associated fibroblasts (prostate))
Loading amount 30 µg
Specification primary cancer-associated fibroblasts (prostate)
Gel Running Conditions Reduced Denaturing (Invitrogen's NuPAGE 4-12%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Alexander Henke

Verified customer

Submitted Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"