Overview

  • Product nameAnti-Hsp90 beta antibody [E296]
    See all Hsp90 beta primary antibodies
  • Description
    Rabbit monoclonal [E296] to Hsp90 beta
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Hsp90 beta aa 1-100 (N terminal).

  • Positive control
    • Hela cell lysate and urinary bladder carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberE296
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab32568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000 - 1/500000. Detects a band of approximately 92 kDa (predicted molecular weight: 83 kDa).

For unpurified, use 1/500.

IHC-P 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
    • Sequence similaritiesBelongs to the heat shock protein 90 family.
    • DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
    • Post-translational
      modifications
      Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
      ISGylated.
      S-nitrosylated; negatively regulates the ATPase activity.
    • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kda heat shock protein beta HSP90 beta antibody
      • D6S182 antibody
      • FLJ26984 antibody
      • Heat shock 84 kDa antibody
      • Heat shock 90kD protein 1, beta antibody
      • Heat shock 90kDa protein 1 beta antibody
      • Heat shock protein 90 alpha family class B member 1 antibody
      • Heat shock protein 90 kDa antibody
      • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
      • Heat shock protein 90kDa alpha family class B member 1 antibody
      • Heat shock protein beta antibody
      • Heat shock protein HSP 90 beta antibody
      • Heat shock protein HSP 90-beta antibody
      • HS90B_HUMAN antibody
      • HSP 84 antibody
      • HSP 90 antibody
      • HSP 90 b antibody
      • HSP 90b antibody
      • HSP84 antibody
      • HSP90 BETA antibody
      • hsp90ab1 antibody
      • HSP90B antibody
      • HSPC2 antibody
      • HSPCB antibody
      see all

    Anti-Hsp90 beta antibody [E296] images

    • All lanes : Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 83 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution (purified) + SH-S5SY cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 83 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 83 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human stomach with purified ab32568 at a working dilution of 1 in 150. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunofluorescence staining of HepG2 cells with purified ab32568 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit (ab150082), used at a dilution of 1 in 400. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab32568 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

    • Anti-Hsp90 beta antibody [E296] (ab32568) at 1/500 dilution (unpurified) + Hela cell lysate

      Predicted band size : 83 kDa
      Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using unpurified ab32568 at 1/50 dilution.

      Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using ab32568 at 1/50 dilution.

    References for Anti-Hsp90 beta antibody [E296] (ab32568)

    This product has been referenced in:
    • Jarboui MA  et al. Nucleolar Protein Trafficking in Response to HIV-1 Tat: Rewiring the Nucleolus. PLoS One 7:e48702 (2012). WB . Read more (PubMed: 23166591) »
    • Ge F  et al. Proteomic and functional analyses reveal a dual molecular mechanism underlying arsenic-induced apoptosis in human multiple myeloma cells. J Proteome Res 8:3006-19 (2009). Read more (PubMed: 19364129) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"