Anti-Hsp90 beta antibody [K3701] (ab82584)
Key features and details
- Mouse monoclonal [K3701] to Hsp90 beta
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Rat, Human, Chinese hamster
- Isotype: IgM
Overview
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Product name
Anti-Hsp90 beta antibody [K3701]
See all Hsp90 beta primary antibodies -
Description
Mouse monoclonal [K3701] to Hsp90 beta -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Rat, Human, Chinese hamster -
Immunogen
Recombinant human Hsp90 beta protein
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Positive control
- WB: A431, Saos-2, HL-60, HeLa, PC12, CHO-K1 cell lysates; Hsp90 native protein. Flow Cyt (Intra): Human colon cancer Caco-2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
K3701 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 83 kDa.
1/1000 (colorimetric). |
Notes |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 83 kDa. 1/1000 (colorimetric). |
Target
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Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. -
Post-translational
modificationsUbiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3326 Human
- Entrez Gene: 301252 Rat
- Omim: 140572 Human
- SwissProt: P08238 Human
- SwissProt: P34058 Rat
- Unigene: 509736 Human
- Unigene: 98667 Rat
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Alternative names
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
see all
Images
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All lanes : Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HSP90AB1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Saos-2 (Human osteosarcoma cell line) whole cell lysate
Lane 4 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab82584 observed at 85 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab82584 was shown to react with Hsp90 beta in wild-type A431 cells in western blot. Loss of signal was observed when HSP90AB1 knockout sample was used. Wild-type A431 and HSP90AB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab82584 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : CHO-K1 cell lysate
Lane 4 : Hsp90 Native Protein
Lane 5 : Hsp90 alpha Recombinant Protein (negative control)
Predicted band size: 83 kDa
Observed band size: 83 kDa -
ab82584, at 10 µg/ml, staining p90 beta in human colon cancer Caco-2 cells by flow cytometry (right). Isotype control antibody (left).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab82584 has not yet been referenced specifically in any publications.