• Product nameAnti-Hsp90 beta antibody [K3705]
    See all Hsp90 beta primary antibodies
  • Description
    Mouse monoclonal [K3705] to Hsp90 beta
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Immunogen

    Recombinant human Hsp90 beta protein

  • Positive control
    • HeLa cells, PC-12 cells, CHO-K1 cells, 3T3 cells. Human breast cancer tissue. IHC-P: Human pancreas FFPE tissue sections.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Saline, pH 6.9
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • ClonalityMonoclonal
  • Clone numberK3705
  • IsotypeIgM
  • Research areas


Our Abpromise guarantee covers the use of ab82522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).


  • FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • Post-translational
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    S-nitrosylated; negatively regulates the ATPase activity.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Anti-Hsp90 beta antibody [K3705] images

  • IHC image of Hsp90 beta staining in human liver formalin fixed paraffin embedded tissue section, using ABC detection kit, ab94264. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was incubated with ab8522, 5µg/ml, for 15 mins at room temperature . A goat anti-mouse biotinylated secondary antibody was used to detect the primary. The section was counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : Hsp90 Native Human Protein
    Lane 3 : Hsp90 alpha Recombinant Human Protein
    Lane 4 : Hsp90 beta Recombinant Human Protein
    Lane 5 : HeLa cells
    Lane 6 : PC-12 cells
    Lane 7 : CHO-K1 cells
    Lane 8 : 3T3 cells

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution + Hamster CHO cell lysate

    Predicted band size : 83 kDa
    Observed band size : 83 kDa

References for Anti-Hsp90 beta antibody [K3705] (ab82522)

This product has been referenced in:
  • Soleilhavoup C  et al. Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. J Proteomics 109C:245-260 (2014). WB ; Sheep . Read more (PubMed: 25053255) »
  • Nemoto T  et al. Domain structures and immunogenic regions of the 90-kDa heat-shock protein (HSP90). Probing with a library of anti-HSP90 monoclonal antibodies and limited proteolysis. J Biol Chem 272:26179-87 (1997). Read more (PubMed: 9334185) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (8-16%)
Sample Sheep Serum (Cervical mucus)
Specification Cervical mucus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Mr. Guillaume TSIKIS

Verified customer

Submitted Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (heart tissue)
Loading amount 50 µg
Specification heart tissue
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 10 2010