Recombinant
RabMAb

Anti-HSPA14 antibody [EPR4210] (ab108612)

Overview

  • Product name
    Anti-HSPA14 antibody [EPR4210]
    See all HSPA14 primary antibodies
  • Description
    Rabbit monoclonal [EPR4210] to HSPA14
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human HSPA14.

  • Positive control
    • K562, COS1, Human fetal liver, and HeLa cell lysates. Human kidney tissue. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4210
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
    • Sequence similarities
      Belongs to the heat shock protein 70 family.
    • Cellular localization
      Cytoplasm > cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock 70 kDa protein 14 antibody
      • Heat shock 70kDa protein 14 antibody
      • Heat shock protein HSP60 antibody
      • Heat shock protein hsp70 related protein antibody
      • HSP60 antibody
      • HSP70 4 antibody
      • HSP70 like protein 1 antibody
      • HSP70-like protein 1 antibody
      • HSP70L1 antibody
      • HSP7E_HUMAN antibody
      • HSPA 14 antibody
      • HSPA14 antibody
      • MGC131990 antibody
      see all

    Images

    • All lanes : Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : COS1 cell lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.
    • Immunofluorescent staining of HeLa cells using ab108612 at 1/100

    References

    ab108612 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108612.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up