Overview

  • Product nameAnti-HSPA1L antibody
    See all HSPA1L primary antibodies
  • Description
    Mouse monoclonal to HSPA1L
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Chimpanzee, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Recombinant fragment: KGKISESDKN KILDKCNELL SWLEVNQLAE KDEFDHKRKE LEQMCNPIIT KLYQGGCTGP ACGTGYVPGR PATGPTIEEV D, corresponding to amino acids 561-641 of Human HSPA1L (NP_005518) with a tag of 26 kDa.

  • Positive control
    • HeLa and PC12 cell lysates. Recombinant tagged human HSPA1L fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
  • Tissue specificityExpressed in spermatids.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heat shock 70 kDa protein 1 Hom antibody
    • Heat shock 70 kDa protein 1 like antibody
    • Heat shock 70 kDa protein 1-Hom antibody
    • Heat shock 70 kDa protein 1-like antibody
    • Heat shock 70 kDa protein 1L antibody
    • Heat shock 70kD protein like 1 antibody
    • HS71L_HUMAN antibody
    • HSP70 1L antibody
    • HSP70 HOM antibody
    • HSP70-Hom antibody
    • HSPA1L antibody
    • hum70t antibody
    • Spermatid specific heat shock protein 70 antibody
    see all

Anti-HSPA1L antibody images

  • Anti-HSPA1L antibody (ab77155) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPA1L antibody (ab77155) at 1 µg/ml + PC12 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPA1L antibody (ab77155) at 1 µg/ml + Recombinant tagged human HSPA1L fragment at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 70 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-HSPA1L antibody (ab77155)

ab77155 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"