Overview

  • Product nameAnti-HSPA2 antibodySee all HSPA2 primary antibodies ...
  • Description
    Rabbit polyclonal to HSPA2
  • SpecificityThe antibody is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73).
  • Tested applicationsWB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Hamster, Dog, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide surrounding amino acid 560 of human HSPA2.

  • General notes


    Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but that are localized within different intracellular compartments. These include a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in cells experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is useful in ascertaining whether a stress response has occurred in the cell. It should be noted however, that there is a constitutive level of Hsp70 expression in some cell types from primates including human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • Primary antibody notes Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but that are localized within different intracellular compartments. These include a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in cells experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is useful in ascertaining whether a stress response has occurred in the cell. It should be noted however, that there is a constitutive level of Hsp70 expression in some cell types from primates including human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab1428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Flow Cyt
  • Application notesELISA: Use at an assay dependent dilution. (from PMID 17545699)
    Flow Cyt: Use at an assay dependent dilution. (from PMID 17545699)
    WB: Use at a concentration of 1 - 2 µg/ml.
    Can be blocked with Hsp70 peptide (ab55800).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
    • Sequence similaritiesBelongs to the heat shock protein 70 family.
    • Information by UniProt
    • Database links
    • Alternative names
      • 70kDa antibody
      • DAQB 147D11.1 001 antibody
      • Hcp70.2 antibody
      • Heat shock 70 kDa protein 2 antibody
      • heat shock 70kDa protein 1A antibody
      • Heat shock protein 2 antibody
      • heat shock protein 70 antibody
      • Heat shock protein 70.2 antibody
      • Heat shock related 70 kDa protein 2 antibody
      • Heat shock-related 70 kDa protein 2 antibody
      • Heat-shock protein, 70-KD, 2 antibody
      • Heat-shock protein, 70-KD, 3 antibody
      • HSP70 2 antibody
      • HSP70 3 antibody
      • Hsp70-2 antibody
      • HSP70-3 antibody
      • HSP70.2 antibody
      • HSP70A2 antibody
      • HSP72 antibody
      • HSP72_HUMAN antibody
      • HSPA2 antibody
      • Hspt70 antibody
      • Hst70 antibody
      • MGC58299 antibody
      • MGC7795 antibody
      • MGC93458 antibody
      • OTTHUMP00000180664 antibody
      • Testis-specific heat shock protein-related antibody
      • XXbac BCX40G17.3 001 antibody
      see all

    References for Anti-HSPA2 antibody (ab1428)

    This product has been referenced in:
    • Opazo MC  et al. Maternal Hypothyroxinemia impairs Spatial Learning and Synaptic nature and function in the Offspring. Endocrinology : (2008). Read more (PubMed: 18566112) »
    • Martin AM  et al. Immune responses to abacavir in antigen-presenting cells from hypersensitive patients. AIDS 21:1233-44 (2007). Read more (PubMed: 17545699) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (8-16 %)
    Sample Sheep Serum (Oviductal Fluid)
    Specification Oviductal Fluid
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Mr. Guillaume TSIKIS

    Verified customer

    Submitted Jul 02 2013

    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS and HeLa cells)
    Loading amount 30 µg
    Specification U2OS and HeLa cells
    Gel Running Conditions Reduced Denaturing (12% Tris Glycine)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 18 2011

    Thank you for your enquiry. I have been in touch with the originator of this product in relation to this product. A band was observed mainly at 70-72 kDa. A very faint band can be seen at ~80 kDa. The 70-72 kDa band is the correct band; however the ide...

    Read More

    Thank you for your enquiry. The antibody is specific for the inducible form of Hsp70 (Hsp72). It does not cross-react with the constitutive Hsc70 (Hsp 73). hope this is helpful. I hope this information helps. Please do not hesitate to contact ...

    Read More

    Thank you for yur enquiry. The immunogen is a synthetic peptide surrounding amino acid 560 of human Hsp70. I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Thank you for your enquiry regarding ab1428 and your interest in our products. The antibody detects only the inducible form of Hsp70 (Hsp72), it does not react with constitutive Hsc70 (Hsp73). I hope that you will find this answer useful.

    The immunogen is a synthetic peptide consisting of a.a. surrounding 556 of human Hsp70.

    Thank you for your enquiry. Ab1428 is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73). To our knowledge, this antibody has yet to be tested in ICC. If you decide to go ahead and purchase thi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"