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Rabbit monoclonal [EPR14165] to HSPA4
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPA4 aa 100-200. The exact sequence is proprietary.
Database link: P34932
K562 cells and cell lysates; HepG2 cell lysate.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/30 - 1/50.
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).
Belongs to the heat shock protein 70 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
APG 2 antibody
Epididymis secretory sperm binding protein Li 5a antibody
Heat shock 70 kDa protein 4 antibody
Western blot - Anti-HSPA4 [EPR14165] antibody (ab185219)
All lanes : Anti-HSPA4 antibody [EPR14165] (ab185219) at 1/5000 dilution Lane 1 : K562 cell lysate Lane 2 : HepG2 cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution Predicted band size : 94 kDa
Flow Cytometry - Anti-HSPA4 [EPR14165] antibody (ab185219)
Flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-HSPA4 [EPR14165] antibody (ab185219)
Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"