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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPA4 aa 100-200. The exact sequence is proprietary.
Database link: P34932
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab185219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/30 - 1/50.|
|WB||1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).|
Flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.
ab185219 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"