Recombinant
RabMAb

Anti-HSPA4 antibody [EPR14165] (ab185219)

Overview

  • Product name
    Anti-HSPA4 antibody [EPR14165]
    See all HSPA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR14165] to HSPA4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPA4 aa 100-200. The exact sequence is proprietary.
    Database link: P34932

  • Positive control
    • K562 cells and cell lysates; HepG2 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30 - 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).

Target

Images

  • All lanes : Anti-HSPA4 antibody [EPR14165] (ab185219) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

  • Flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.

References

ab185219 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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