Overview

  • Product name
  • Description
    Rabbit polyclonal to HSPA4L
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (CHAEHTPEEE IDHTGAKTKS AVSDKQDRLN QTLKKGKVKS IDLPIQSSLC) of human HSPA4L (NP_055093).

  • Positive control
    • COLO205 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab87241 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    The HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis.
  • Cellular localization
    Cytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock
  • Database links
  • Alternative names
    • 94kDa antibody
    • AI461691 antibody
    • APG 1 antibody
    • Heat shock 70kDa protein 4 like antibody
    • MGC117843 antibody
    • MGC187594 antibody
    • Osmotic stress protein 94 antibody
    • Osp94 antibody
    • OTTMUSP00000027739 antibody
    see all

Anti-HSPA4L antibody images

  • Anti-HSPA4L antibody (ab87241) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa

References for Anti-HSPA4L antibody (ab87241)

ab87241 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse breast tumor)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1e+006 cells
Specification
Mouse breast tumor
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 15 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colorectal cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Treatment
siRNA (control and HSPA4L)
Specification
HCT116 colorectal cancer cell line
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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