Overview

  • Product nameAnti-HSPA4L antibody
    See all HSPA4L primary antibodies
  • Description
    Rabbit polyclonal to HSPA4L
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (CHAEHTPEEE IDHTGAKTKS AVSDKQDRLN QTLKKGKVKS IDLPIQSSLC) of human HSPA4L (NP_055093).

  • Positive control
    • COLO205 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab87241 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis.
  • Cellular localizationCytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock
  • Database links
  • Alternative names
    • 94kDa antibody
    • AI461691 antibody
    • APG 1 antibody
    • Heat shock 70kDa protein 4 like antibody
    • MGC117843 antibody
    • MGC187594 antibody
    • Osmotic stress protein 94 antibody
    • Osp94 antibody
    • OTTMUSP00000027739 antibody
    see all

Anti-HSPA4L antibody images

  • Anti-HSPA4L antibody (ab87241) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa

References for Anti-HSPA4L antibody (ab87241)

ab87241 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HCT116 colorectal cancer cell line)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 30 µg
Treatment siRNA (control and HSPA4L)
Specification HCT116 colorectal cancer cell line
Blocking step Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"