Overview

  • Product name
  • Description
    Rabbit polyclonal to HSPA4L
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C-terminus of Human HSPA4L (NP_055093.2)

  • Positive control
    • Hela cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 94 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis.
    • Cellular localization
      Cytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock
    • Database links
    • Alternative names
      • 94kDa antibody
      • AI461691 antibody
      • APG 1 antibody
      • Heat shock 70kDa protein 4 like antibody
      • MGC117843 antibody
      • MGC187594 antibody
      • Osmotic stress protein 94 antibody
      • Osp94 antibody
      • OTTMUSP00000027739 antibody
      see all

    Images

    • Anti-HSPA4L antibody (ab93348) at 1/100 dilution + HeLa cell lysate at 35 µg

      Predicted band size : 94 kDa

    References

    ab93348 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse breast tumor)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    1e+006 cells
    Specification
    Mouse breast tumor
    Blocking step
    Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 23 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 (colorectal cancer cell line))
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    30 µg
    Treatment
    siRNA targeting HSPA4L
    Specification
    HCT116 (colorectal cancer cell line)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 05 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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