Anti-HSPA6 antibody (ab212044)
Key features and details
- Rabbit polyclonal to HSPA6
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSPA6 antibody
See all HSPA6 primary antibodies -
Description
Rabbit polyclonal to HSPA6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human HSPA6 aa 377-628. The exact sequence is proprietary.
Database link: P17066 -
Positive control
- Human heart tissue; A431 cells; H1299 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
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WB |
1/500 - 1/3000. Predicted molecular weight: 71 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
WB
1/500 - 1/3000. Predicted molecular weight: 71 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. -
Sequence similarities
Belongs to the heat shock protein 70 family. - Information by UniProt
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Database links
- Entrez Gene: 3310 Human
- Omim: 140555 Human
- SwissProt: P17066 Human
- Unigene: 654614 Human
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Alternative names
- Heat shock 70 kDa protein 6 antibody
- Heat shock 70 kDa protein B' antibody
- Heat shock 70 kDa protein B'' antibody
see all
Images
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Anti-HSPA6 antibody (ab212044) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 71 kDa7.5% SDS PAGE
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human heart tissue labeling HSPA6 with ab212044 at 7.5 µg/ml.
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Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling HSPA6 with ab212044 at 1/200 dilution (green). The lower panel is merged with DNA probe.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab212044 has been referenced in 3 publications.
- Jiang W et al. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs. iScience 25:104504 (2022). PubMed: 35754733
- Chi L et al. N-Acetyl-L-Cysteine Protects Airway Epithelial Cells during Respiratory Syncytial Virus Infection against Mucin Synthesis, Oxidative Stress, and Inflammatory Response and Inhibits HSPA6 Expression. Anal Cell Pathol (Amst) 2022:4846336 (2022). PubMed: 36046596
- Zhang LH et al. Proteomic signatures of infiltrative gastric cancer by proteomic and bioinformatic analysis. World J Gastrointest Oncol 14:2097-2107 (2022). PubMed: 36438703