Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 4 ARM repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1500019G21Rik antibody
    • FES1 antibody
    • Heat shock protein 70 binding protein antibody
    • Heat shock protein 70 interacting protein antibody
    • Heat shock protein binding protein 1 antibody
    • Heat shock protein-binding protein 1 antibody
    • Heat-shock 70-KD protein-binding protein 1 antibody
    • HPBP1_HUMAN antibody
    • Hsp 70 binding protein antibody
    • Hsp 70 interacting protein antibody
    • Hsp70 binding protein 1 antibody
    • Hsp70 binding protein 2 antibody
    • Hsp70 interacting protein 1 antibody
    • Hsp70 interacting protein 2 antibody
    • Hsp70-binding protein 1 antibody
    • Hsp70-binding protein 2 antibody
    • Hsp70-interacting protein 1 antibody
    • Hsp70-interacting protein 2 antibody
    • HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 antibody
    • HSPA-binding protein 1 antibody
    • HSPBP antibody
    • HspBP1 antibody
    • HspBP2 antibody
    • PP1845 antibody
    see all

Images

  • Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
    Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution

    Lane 1 : MCF-7 Whole Cell Lysate
    Lane 2 : MCF-7 Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39.3 kDa
    Observed band size : 39.3 kDa


    Exposure time : 2 minutes
  • Image courtesy of Human Protein Atlas

    Paraffin embedded sections of human kidney tissue were incubated with ab3858 (1/75 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab3858 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org

  • ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.

References

ab3858 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

This antibody has been tested briefly in Immunofluorescence in U20S cells (human). IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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