HspBP1 overexpression 293T lysate (whole cell) (ab94320)

Overview

  • Product nameHspBP1 overexpression 293T lysate (whole cell)
  • Description
    HspBP1 overexpression lysate
  • General notesab94320 is a 293T cell transfected lysate in which Human HspBP1 has been transiently over-expressed using a pCMV-HspBP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. Tissue specificity: Ubiquitous. Similarity: Contains 4 ARM repeats.

Applications

Our Abpromise guarantee covers the use of ab94320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

HspBP1 overexpression 293T lysate (whole cell) images

  • ab94320 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HspBP1 antibody (ab88826) at 1/500 dilution

    Lane 1 : HspBP1 overexpression 293T lysate (whole cell) (ab94320)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 at 1/2500 dilution

References for HspBP1 overexpression 293T lysate (whole cell) (ab94320)

ab94320 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"