Overview

  • Product name
    Anti-HSPC014 antibody [EPR10177]
    See all HSPC014 primary antibodies
  • Description
    Rabbit monoclonal [EPR10177] to HSPC014
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human HSPC014 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y244

  • Positive control
    • HeLa, SW480, HL60 and Jurkat cell lysates; Human skin tissue; Permeabilized Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.
    • Tissue specificity
      Strongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer.
    • Involvement in disease
      Defects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) [MIM:601952]. KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer.
    • Sequence similarities
      Belongs to the POMP/UMP1 family.
    • Cellular localization
      Cytoplasm > cytosol. Nucleus. Microsome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2510048O06Rik antibody
      • C13orf12 antibody
      • Chromosome 13 open reading frame 12 antibody
      • HSPC 014 antibody
      • HSPC036 protein antibody
      • hUMP 1 antibody
      • hUMP1 antibody
      • PNAS 110 antibody
      • PNAS110 antibody
      • Pomp antibody
      • POMP_HUMAN antibody
      • Proteasome maturation protein antibody
      • Proteassemblin antibody
      • Protein UMP1 homolog antibody
      • UMP 1 antibody
      • UMP1 antibody
      • UMP1, yeast, homolog of antibody
      • Voltage gated K channel beta subunit 4.1 antibody
      • Voltage-gated K channel beta subunit 4.1 antibody
      • voltage-gated potassium channel beta subunit 4.1 antibody
      see all

    Images

    • Detection of HSPC014 by Western Blot of Immunprecipitate.
      HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2)  and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Flow cytometric analysis of permeabilized Jurkat cells labeling HSPC014 with ab170865 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling HSPC014 with ab170865 at 1/50 dilution.

    • All lanes : Anti-HSPC014 antibody [EPR10177] (ab170865) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : HL60 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 16 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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