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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HSPC014 (Cysteine residue).
Database link: Q9Y244
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab170865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 16 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Detection of HSPC014 by Western Blot of Immunprecipitate.
HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized Jurkat cells labeling HSPC014 with ab170865 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling HSPC014 with ab170865 at 1/50 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"