Overview

  • Product name
  • Description
    Mouse polyclonal to HSPC111
  • Specificity
    Reactive against mammalian transfected lysate
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Human HSPC111 (AAH40106.1)

  • Positive control
    • WB: HeLa, PC-12, Raw 264.7 or NIH/3T3 cell lysate Transfected 293T cell lysate IHC-P: Human esophagus tissue IF/ICC: HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

Images

  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + PC-12 cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HSPC111 antibody (ab88449) at 1 µg/ml

    Lane 1 : Transfected 293T lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • ab88449 at 3µg/ml, staining HSPC111 in Human esophagus, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • Immunofluorescence image staining HSPC111 in HeLa cells. ab88449 was used at 10 ug/ml.

References

This product has been referenced in:
  • Kundel DW  et al. Molecular characterizations of Nop16 in murine mammary tumors with varying levels of c-Myc. Transgenic Res 21:393-406 (2012). Read more (PubMed: 21863248) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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