Overview

  • Product name
  • Description
    Rabbit polyclonal to HSPC142
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 175 and 225 of Human HSPC142 (NP_054892.2)

  • Positive control
    • Human breast carcinoma and osteosarcoma tissues

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Target

  • Function
    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
  • Sequence similarities
    Belongs to the BABAM1 family.
  • Domain
    The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • BABA1_HUMAN antibody
    • babam1 antibody
    • BRCA1 A complex subunit MERIT40 antibody
    • BRISC and BRCA1-A complex member 1 antibody
    • C19orf62 antibody
    • FLJ20571 antibody
    • HSPC142 antibody
    • hypothetical protein LOC29086 antibody
    • Mediator of Rap80 interactions and targeting 40 kDa antibody
    • Mediator of RAP80 interactions and targeting subunit of 40 kDa antibody
    • MERIT 40 antibody
    • MERIT40 antibody
    • NBA1 antibody
    • New component of the BRCA1 A complex antibody
    • New component of the BRCA1-A complex antibody
    • Uncharacterized protein C19orf62 antibody
    see all

Images

  • ab124647, at 1/250 dilution, staining HSPC142 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry with DAB staining.

References

ab124647 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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