General notesPrincipal Names – HSPC150; HSPC150 protein similar to ubiquitin-conjugating enzyme.
Official Gene Symbol - HSPC150 (interim symbol).
GenBank Accession Number – NP_054895.
LocusLink ID - 29089 (human).
Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; protein modification; ligase activity.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 22.5 kDa).Can be blocked with Human HSPC150 peptide (ab23018).
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.