The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionParticipates both in methylation of protein and tRNA species. The heterodimer with HEMK2/N6AMT1 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species.
Sequence similaritiesBelongs to the TRM112 family. Contains 1 TRM112 domain.
tRNA methyltransferase 11-2 homolog (S. cerevisiae) antibody
tRNA methyltransferase 112 homolog antibody
Anti-HSPC152 antibody images
Western blot - HSPC152 antibody (ab57156)
Western blot against tagged recombinant protein immunogen using ab57156 HSPC152 antibody at 1ug/ml. Predicted band size of immunogen is 40 kDa
References for Anti-HSPC152 antibody (ab57156)
This product has been referenced in:
Fu D et al. Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival. Mol Cell Biol30:2449-59 (2010).
Read more (PubMed: 20308323) »