HsPGK1 ChIP primer Exon 1 (Forward) (ab83594)

Overview

  • Product name
    HsPGK1 ChIP primer Exon 1 (Forward)
    See all PGK1 dna rna and cdna
  • General notes

    Accession number: NM_000291.The HsPGK1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. PGK1 (3-phosphoglycerate kinase 1) is a highly expressed housekeeping gene located on the X chromosome. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most female cells the locus is enriched with both active (e.g. Histone H3 K18 acetylation, see image with ab1191). and heterochromatic (e.g. Histone H3 K9 tri methylation, see image with ab8898) histone modification marks, as one copy of the gene undergoes X chromosome inactivation. In male cells, this locus is highly enriched with histone modifications associated with active gene transcription whereas it only shows low levels of heterochromatic histone modifications.

  • Tested applications
    Suitable for: Real Time PCRmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab83594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Real Time PCR Use at an assay dependent concentration.

Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

References

ab83594 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab83594.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up