Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)

Overview

  • Product nameAnti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]
  • Description
    Mouse monoclonal [10F1] to HSV 1 ICP4 Immediate Early Protein
  • SpecificityELISA titer 1/25,600 absorption of 0.10
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Herpes Virus

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPhosphate buffered saline
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number10F1
  • MyelomaNS1/1-Ag4-1
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/800.
ELISA 1/25600.
ICC/IF 1/25600.

Target

  • RelevanceThis IE protein is a multifunctional protein capable of migrating to the nucleus, binding to DNA, trans-activating other viral genes, and autoregulating its own synthesis. Binding of ICP4 to alpha genes causes repression of their expression. ICP4 also acts as a transcriptional activator of beta and gamma genes.
  • Cellular localizationNuclear
  • Alternative names
    • Herpes simplex virus 1 ICP4 antibody
    • HSV RS1 antibody
    • ICP4 antibody
    • Immediate early protein antibody
    • RS1 antibody
    • Transcriptional regulator ICP4 antibody
    see all

Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] images

  • ab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in mouse 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.

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  • All lanes : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution

    Lane 1 : HeLa cells (whole cell lysate) - mock infection, cytosolic fraction
    Lane 2 : HeLa cells (whole cell lysate) - mock infection, membrane fraction
    Lane 3 : HeLa cells (whole cell lysate) - mock infection, nuclear fraction
    Lane 4 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction
    Lane 5 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction
    Lane 6 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction
    Lane 7 : HeLa cells (whole cell lysate) - mock infected
    Lane 8 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated sheep anti-mouse IgG

    Observed band size : 97 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

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References for Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)

This product has been referenced in:
  • Abe T & Barber GN Cytosolic-DNA-mediated, STING-dependent proinflammatory gene induction necessitates canonical NF-?B activation through TBK1. J Virol 88:5328-41 (2014). Read more (PubMed: 24600004) »
  • Bolyard C  et al. Doxorubicin synergizes with 34.5ENVE to enhance antitumor efficacy against metastatic ovarian cancer. Clin Cancer Res 20:6479-94 (2014). Read more (PubMed: 25294909) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Feb 11 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 10 µg
Specification HeLa cells
Treatment Infected with HSV KOS for 16 hours
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Vero Cells)
Loading amount 10 µg
Specification Vero Cells
Treatment Infected with HSV KOS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Aug 14 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa adherent cells)
Specification HeLa adherent cells
Fixative Formaldehyde
Permeabilization Yes - 0.5 % NP40
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Jul 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (293T cells, kidney epithelium)
Specification 293T cells, kidney epithelium
Fixative Formaldehyde
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Verified customer

Submitted Jul 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"