Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)

Overview

  • Product name
    Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]
  • Description
    Mouse monoclonal [10F1] to HSV 1 ICP4 Immediate Early Protein
  • Specificity
    ELISA titer 1/25,600 absorption of 0.10
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Herpes Virus

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    10F1
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/800.
ELISA 1/25600.
ICC/IF 1/25600.

Target

  • Relevance
    This IE protein is a multifunctional protein capable of migrating to the nucleus, binding to DNA, trans-activating other viral genes, and autoregulating its own synthesis. Binding of ICP4 to alpha genes causes repression of their expression. ICP4 also acts as a transcriptional activator of beta and gamma genes.
  • Cellular localization
    Nuclear
  • Alternative names
    • Herpes simplex virus 1 ICP4 antibody
    • HSV RS1 antibody
    • ICP4 antibody
    • Immediate early protein antibody
    • RS1 antibody
    • Transcriptional regulator ICP4 antibody
    see all

Images

  • Lane 1 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/200 dilution
    Lane 2 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/400 dilution
    Lane 3 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/800 dilution
    Lane 4 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1600 dilution

    Lane 1 : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract
    Lane 2 : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract
    Lane 3 : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract
    Lane 4 : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    Anti-Mouse secondary at 1/5000 dilution
  • All lanes : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution

    Lane 1 : HeLa cells (whole cell lysate) - mock infection, cytosolic fraction
    Lane 2 : HeLa cells (whole cell lysate) - mock infection, membrane fraction
    Lane 3 : HeLa cells (whole cell lysate) - mock infection, nuclear fraction
    Lane 4 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction
    Lane 5 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction
    Lane 6 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction
    Lane 7 : HeLa cells (whole cell lysate) - mock infected
    Lane 8 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated sheep anti-mouse IgG

    Observed band size : 97 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    As shown, ICP4 is detected mainly in the nuclear fraction of infected cells (lane 6). The presence of ICP4 in the cytosolic fraction (lane 4) probably represents the protein undergoing synthesis.

    See Abreview

  • ab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in mouse 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • El Bilali N  et al. Quantitative Evaluation of Protein Heterogeneity within Herpes Simplex Virus 1 Particles. J Virol 91:N/A (2017). WB . Read more (PubMed: 28275191) »
  • Millet R  et al. Impact of the MRN Complex on Adeno-Associated Virus Integration and Replication during Coinfection with Herpes Simplex Virus 1. J Virol 89:6824-34 (2015). Read more (PubMed: 25903339) »

See all 18 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Treatment
Infected with HSV KOS for 16 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero Cells)
Loading amount
10 µg
Specification
Vero Cells
Treatment
Infected with HSV KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 14 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa adherent cells)
Specification
HeLa adherent cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.5 % NP40
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (293T cells, kidney epithelium)
Specification
293T cells, kidney epithelium
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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