Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
ELISA 1/100 - 1/500.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    HSV is a peptide epitope tag, sequence QPELAPEDPED.
  • Alternative names
    • HSV epitope tag antibody
    • QPELAPEDPED tag antibody

Images

  • ab19354 staining mouse embryonic brain tissue sections by IHC-P.  Sections were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C.  The primary antibody was diluted 1/400 and incubated with the sample for 16 hours at 4°C.  A Cy3® conjugated donkey anti-goat antibody was used as the secondary.

    See Abreview

  • ab19354 staining HSV tag in the Human HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 4°C. A FITC-conjugated Donkey anti-Goat polyclonal(1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Wong FK  et al. Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex. PLoS Biol 13:e1002217 (2015). IF . Read more (PubMed: 26252244) »
  • Padilla BE  et al. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J Cell Biol 179:981-97 (2007). Flow Cyt, IP . Read more (PubMed: 18039931) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Human Purified protein (kidney)
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (Live yeast staining on surface displayed protein)
Specification
Live yeast staining on surface displayed protein
Preparation
Cell harvesting/tissue preparation method: spin down and wash with water
Sample buffer: 1XPBS
Permeabilization
No
Gating Strategy
HSV expression positive
Username

Dr. Lei Chen

Verified customer

Submitted Aug 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryonic brain)
Specification
Embryonic brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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