Purification notesAntibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to fluorescein isothiocyanate (FITC).
Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/400.
RelevanceHSV is a peptide epitope tag, sequence QPELAPEDPED.
HSV epitope tag antibody
QPELAPEDPED tag antibody
Anti-HSV tag antibody (FITC) images
Immunocytochemistry/ Immunofluorescence - Anti-HSV tag antibody (FITC) (ab19397)Image from Padilla BE et al, J Cell Biol. 2007 Dec 3;179(5):981-97. Epub 2007 Nov 26, Fig 7. DOI 10.1083/jcb.200704053.
HEK cells expressing ECE-1a-d-HSV were incubated with ab19397 for 1 hour at 4°C, washed, and incubated for 30 minutes at 37°C. After warming, ECE-1a-d trafficked from the plasma membrane (arrowheads) to endosomes (arrows).
References for Anti-HSV tag antibody (FITC) (ab19397)
This product has been referenced in:
Padilla BE et al. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J Cell Biol179:981-97 (2007).
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