Customer reviews and FAQs

Overview

  • Product name
    Anti-HSV1 + HSV2 gD antibody [2C10]
  • Description
    Mouse monoclonal [2C10] to HSV1 + HSV2 gD
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Herpes Virus

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2C10
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000.
ELISA 1/102400.
ICC/IF 1/3200 - 1/25600.

1/3,200 (HSV-2) - 1/25,600 (HSV-1).

Target

  • Relevance
    Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body. Under a microscope, HSV- 1 and 2 are virtually identical, sharing approximately 50% of their DNA. Both types infect the body's mucosal surfaces, usually the mouth or genitals, and then establish latency in the nervous system. HSV-1 is commonly associated with herpes outbreaks of the face known as cold sores or fever blisters, whereas HSV-2 is more often associated with genital herpes. Herpes simplex viruses (HSV) use multiple and sequential receptors to enter host cells. HSV glycoprotein D (gD) has been implicated in binding to cellular receptors that facilitate virus penetration into cells. Herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) is an essential component of the entry apparatus that is responsible for viral penetration and subsequent cell-cell spread.
  • Alternative names
    • Envelope glycoprotein D antibody
    • GD antibody
    • Glycoprotein D antibody
    • US6 antibody

Anti-HSV1 + HSV2 gD antibody [2C10] images

References for Anti-HSV1 + HSV2 gD antibody [2C10] (ab6507)

This product has been referenced in:
  • Çuburu N  et al. Topical herpes simplex virus 2 (HSV-2) vaccination with human papillomavirus vectors expressing gB/gD ectodomains induces genital-tissue-resident memory CD8+ T cells and reduces genital disease and viral shedding after HSV-2 challenge. J Virol 89:83-96 (2015). WB . Read more (PubMed: 25320297) »
  • Angelova M  et al. Inhibition of O-Linked N-Acetylglucosamine Transferase Reduces Replication of Herpes Simplex Virus and Human Cytomegalovirus. J Virol 89:8474-83 (2015). WB, SDS-PAGE . Read more (PubMed: 26041297) »

See all 8 Publications for this product

Publishing research using ab6507? Please let us know so that we can cite the reference in this datasheet.

Product Wall

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5 Abreviews or Q&A

10
Votes: Highest First

Product code: 43046
Lot number: GR41258-1
Inquiry: Hello, I was using your HSV2 gD recombinant protein as a positive control and used 100 ng on a 4-12% Bis-Tris reducing gel and got no signal. I used your ab6507 antibody at 1:1000 as my prima...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thanks for your reply. Here are the details of the protocol that I followed: Guinea pig fibroblast cells were infected with a recombinant virus with the gene for HSV2gD (from strain 333), HSV1 strain 17Syn+, and HSV2 (strain HG52). The positive c...

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Thank you for providing these details. When this antibody was characterized in Western blotting with HSV-1 Antigen and the HSV-2 Infected Cell Extract, the antibody worked at dilutions from 1:10,000-1:80,000. As you're seeing a very weak signal using a...

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Customer is using this antibody in WB with recombinant HSV2gd (strain 333) and after a one hour exposure saw a very faint band at approx 55 kDa. Primary was used at 1:500 and incubated for 1 hr at RT. She also used HSV2 control (strain Hg52) and go...

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Thank you for your enquiry. I was able to obtain the following information from our source for this antibody about how it was tested in Western blotting. Ab6507 was tested using the HSV-1 Antigen and the HSV-2 Infected Cell Extract at 10 micrograms/cm....

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We have recently purchased a batch of ab6507 antibody from Abcam (order number 111265). Despite the fact that this antibody used to work fairly well in ELISA in the past (please attached see our recent article where we cited Abcam), this new batch of t...

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Thank you for your e-mail and congratulations on your paper. I can certainly arrange a refund on your recent order of ab6507 if the antibody was purchased in the last 90days and if you could provide your order details. We have not received any other...

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Dear Technical expert: I would like to do immunofluorescence using your mouse monoclonal to HSV gD --ab6507 (clone # 2C10). Which fixatives are compatible with this antibody, and which of these is prefered? (i.e. 4% Paraformaldehyde in PHEM OR ...

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I assume that you are doing frozen section work. 100% acetone for 10 minutes is usually a good fixative for this purpose. Aside, use PBS to dilute the antibody and for washes.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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