Anti-HSV1+ HSV2 ICP27 antibody [1-L-11] (ab31631)

Overview

  • Product name
    Anti-HSV1+ HSV2 ICP27 antibody [1-L-11]
    See all HSV1+ HSV2 ICP27 primary antibodies
  • Description
    Mouse monoclonal [H1113] to HSV1+ HSV2 ICP27
  • Specificity
    Recognizes ICP27 of HSV I & II. Reactive in IFA of infected cells and immunoprecipitation of specific viral protein. The clone number has been updated from (8.F.137B) to (H1113) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with Herpes Simplex Virus Not yet tested in other species.
  • Immunogen

    Herpes Simplex Regulatory ICP27.

  • General notes
    The Herpes Simplex Virus 1 protein ICP27 contains 512 amino acids and has an apparent molecular weight of 63kDa.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: 40% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    H1113
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 10 µg/ml.

Target

  • Relevance
    Herpes simplex virus (HSV) ICP27 (Infected cell polypeptide 27) is an essential and multifunctional regulator of viral gene expression that modulates RNA splicing, polyadenylation, and nuclear export. ICP27 Belongs to the herpesviruses UL69 family. It is involved in the down-regulation of viral immediate-early genes and acts in combination with ICP0 and ICP4 as an activator of late genes.
  • Alternative names
    • ICP47 protein antibody
    • Immediate early protein IE12 antibody
    • Infected cell protein 47 antibody
    • US12 antibody
    • US12 protein antibody
    see all

Anti-HSV1+ HSV2 ICP27 antibody [1-L-11] images

  • All lanes : Anti-HSV1+ HSV2 ICP27 antibody [1-L-11] (ab31631) at 1/1000 dilution

    Lane 1 : Mock-infected HeLa cells
    Lane 2 : HeLa cells infected with wt HSV1 virus (KOS strain, MOI of 2) for 16 hours

    Secondary
    Donkey anti-mouse IgG conjugated to HRP at 1/3000 dilution

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-HSV1+ HSV2 ICP27 antibody [1-L-11] (ab31631)

This product has been referenced in:
  • Hu Z  et al. Aqueous synthesized quantum dots interfere with the NF-?B pathway and confer anti-tumor, anti-viral and anti-inflammatory effects. Biomaterials 108:187-96 (2016). Read more (PubMed: 27639114) »
  • Angelova M  et al. Inhibition of O-Linked N-Acetylglucosamine Transferase Reduces Replication of Herpes Simplex Virus and Human Cytomegalovirus. J Virol 89:8474-83 (2015). WB, SDS-PAGE . Read more (PubMed: 26041297) »

See all 13 Publications for this product

Product Wall

The immunogen used to raise the anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631) was HSV-1(HFEM)tsLB2 infected cell extract. The antibody recognizes ICP27 in HSVI and HSVII. No further epitope mapping has been carried out.

Thank you for your inquiry. Unfortunately, we do not have any ICP27 protein available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero cells)
Loading amount
10 µg
Specification
Vero cells
Treatment
Infected with HSV1 KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Treatment
infected with HSV1 KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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