Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631)

Overview

  • Product nameAnti-HSV1+ HSV2 ICP27 antibody [8.F.137B]
    See all HSV1+ HSV2 ICP27 primary antibodies
  • Description
    Mouse monoclonal [8.F.137B] to HSV1+ HSV2 ICP27
  • SpecificityRecognizes ICP27 of HSV I & II. Reactive in IFA of infected cells and immunoprecipitation of specific viral protein.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with Herpes Simplex Virus Not yet tested in other species.
  • Immunogen

    Herpes Simplex Regulatory ICP27.

  • General notesThe Herpes Simplex Virus 1 protein ICP27 contains 512 amino acids and has an apparent molecular weight of 63kDa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: 40% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number8.F.137B
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 10 µg/ml.

Target

  • RelevanceHerpes simplex virus (HSV) ICP27 (Infected cell polypeptide 27) is an essential and multifunctional regulator of viral gene expression that modulates RNA splicing, polyadenylation, and nuclear export. ICP27 Belongs to the herpesviruses UL69 family. It is involved in the down-regulation of viral immediate-early genes and acts in combination with ICP0 and ICP4 as an activator of late genes.
  • Alternative names
    • ICP47 protein antibody
    • Immediate early protein IE12 antibody
    • Infected cell protein 47 antibody
    • US12 antibody
    • US12 protein antibody
    see all

Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] images

  • All lanes : Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631) at 1/1000 dilution

    Lane 1 : Mock-infected HeLa cells
    Lane 2 : HeLa cells infected with wt HSV1 virus (KOS strain, MOI of 2) for 16 hours

    Secondary
    Donkey anti-mouse IgG conjugated to HRP at 1/3000 dilution

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631)

This product has been referenced in:
  • Hu Z  et al. Aqueous synthesized quantum dots interfere with the NF-?B pathway and confer anti-tumor, anti-viral and anti-inflammatory effects. Biomaterials 108:187-96 (2016). Read more (PubMed: 27639114) »
  • Conwell SE  et al. Identification of TRIM27 as a novel degradation target of herpes simplex virus 1 ICP0. J Virol 89:220-9 (2015). WB . Read more (PubMed: 25320289) »

See all 12 Publications for this product

Product Wall

The immunogen used to raise the anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631) was HSV-1(HFEM)tsLB2 infected cell extract. The antibody recognizes ICP27 in HSVI and HSVII. No further epitope mapping has been carried out.

Thank you for your inquiry. Unfortunately, we do not have any ICP27 protein available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Vero cells)
Loading amount 10 µg
Specification Vero cells
Treatment Infected with HSV1 KOS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 10 µg
Specification HeLa cells
Treatment infected with HSV1 KOS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"