The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution (PMID 18701599).
IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200
ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400
Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans.
They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body.
Capsid protein VP5 antibody
Herpes simplex virus 1 major capsid protein antibody
Herpes simplex virus 2 major capsid protein antibody
Human Herpes virus antibody
Major capsid protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] (ab6508)This image is courtesy of an anonymous Abreview
ab6508 staining HSV1 + HSV2 ICP5 Major Capsid Protein in Human head and neck cancer tissue and Mouse xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 1 hour at 23°C; antigen retrieval was by heat mediation in a 10mM sodium citrate, pH6. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] (ab6508)Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. doi: 10.1186/1743-422X-8-365.; Fig 4.; 26 July 2011, Virology Journal 2011, 8:365
Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.
Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.
Ott M et al. Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions. Virol J13:175 (2016).
Read more (PubMed: 27765046) »
Katzenell S & Leib DA Herpes Simplex Virus and Interferon Signaling Induce Novel Autophagic Clusters in Sensory Neurons. J Virol90:4706-19 (2016).
Read more (PubMed: 26912623) »