The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at an assay dependent dilution (PMID 18701599).
IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200
ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400
Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHerpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans.
They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body.
Capsid protein VP5 antibody
Herpes simplex virus 1 major capsid protein antibody
Herpes simplex virus 2 major capsid protein antibody
Human Herpes virus antibody
Major capsid protein antibody
Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] (ab6508)This image is courtesy of an anonymous Abreview
ab6508 staining HSV1 + HSV2 ICP5 Major Capsid Protein in Human head and neck cancer tissue and Mouse xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 1 hour at 23°C; antigen retrieval was by heat mediation in a 10mM sodium citrate, pH6. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] (ab6508)Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. doi: 10.1186/1743-422X-8-365.; Fig 4.; 26 July 2011, Virology Journal 2011, 8:365
Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.
Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.
References for Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] (ab6508)
This product has been referenced in:
Lau SY & Crump CM HSV-1 gM and the gK/pUL20 Complex Are Important for the Localization of gD and gH/L to Viral Assembly Sites. Viruses7:915-38 (2015).
Read more (PubMed: 25746217) »
Zhong M et al. Heat-shock protein 90 promotes nuclear transport of herpes simplex virus 1 capsid protein by interacting with acetylated tubulin. PLoS One9:e99425 (2014).
Read more (PubMed: 24901434) »