Overview

  • Product name
    Anti-HSV1 ICP0 antibody [5H7]
  • Description
    Mouse monoclonal [5H7] to HSV1 ICP0
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Herpes Virus

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    5H7
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000.
ELISA 1/12800.
ICC/IF 1/25600.

Target

  • Relevance
    Herpes simplex virus (HSV) encodes four nuclear phosphoproteins that contribute towards the full productive lytic cycle cascade in newly infected permissive cells. The IE175 (or ICP4) and IE63 (or ICP27) proteins have essential regulatory roles during the lytic cycle. A third, IE110 (or ICP0), appears to play only an accessory role in the presence of VP16, but in the absence of VP16 appears to be critical for initiation of reactivated infections.
  • Cellular localization
    Nuclear
  • Alternative names
    • Alpha 0 protein antibody
    • Herpes siplex virus 1 antibody
    • HHV1 ICP0 antibody
    • HSV1 IE110 antibody
    • Human Herpesvirus 1 antibody
    • IE110 antibody
    • Immediate early nuclear phosphoprotein antibody
    • Immediate early protein IE110 antibody
    • Trans acting transcriptional protein ICP0 antibody
    • VMW110 antibody
    see all

Anti-HSV1 ICP0 antibody [5H7] images

  • ab6513 (1/25600) staining HSV1 ICP0 in human cells. Cells were PFA-fixed, permeabilized in 0.3% TritonX-100/PBS and blocked in 1% serum /30 minutes /25oC. A fluorescently labeled donkey anti-mouse antibody was used as the secondary.

    See Abreview

References for Anti-HSV1 ICP0 antibody [5H7] (ab6513)

This product has been referenced in:
  • Jurak I  et al. Herpes simplex virus is equipped with RNA- and protein-based mechanisms to repress expression of ATRX, an effector of intrinsic immunity. J Virol 86:10093-102 (2012). Read more (PubMed: 22787211) »
  • Dufour F  et al. The ribonucleotide reductase R1 subunits of herpes simplex virus 1 and 2 protect cells against poly(I · C)-induced apoptosis. J Virol 85:8689-701 (2011). WB . Read more (PubMed: 21697465) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HSV1 infected or transfected with plasmid)
Specification
HSV1 infected or transfected with plasmid
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 12 2007

According to the datasheet, this antibody has not been tested for immunohistochemistry. I have looked through our catalogue and am very sorry to inform you that we currently do not stock products that suit to your requirement. If you cannot find...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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