The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
Use at an assay dependent dilution.
Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1.
Herpes simplex virus type 1 DBP antibody
HSV1 DBP antibody
Infected cell protein 8 antibody
Major DNA binding protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (ab20193)This image is courtesy of an anonymous Abreview
ab20193 staining HSV1 ICP8 Major DNA binding protein in Human U2OS by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Franzoso FD et al. Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1. J Virol91:N/A (2017).
Read more (PubMed: 28515305) »
Vogel R et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol86:143-55 (2012).
Read more (PubMed: 22013059) »