Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)

Overview

  • Product nameAnti-HSV1 ICP8 Major DNA binding protein antibody [11E2]
    See all HSV1 ICP8 Major DNA binding protein primary antibodies
  • Description
    Mouse monoclonal [11E2] to HSV1 ICP8 Major DNA binding protein
  • Tested applicationsSuitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with HSV1. Not yet tested in other species.
  • Immunogen

    Full length native protein (purified from U-35-VERO cells) (HSV).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number11E2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 18434395).
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHerpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1.
    • Alternative names
      • DBP antibody
      • Herpes simplex virus type 1 DBP antibody
      • HSV1 DBP antibody
      • ICP8 antibody
      • Infected cell protein 8 antibody
      • Major DNA binding protein antibody
      • UL29 antibody
      see all

    Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] images

    • ab20194 staining HSV1 ICP8 Major DNA binding protein in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunofluorescence analysis of cells infected with HSV1, staining HSV1 ICP8 Major DNA binding protein (purple) with ab20194, 7 (left) or 17 (right) hours after infection.

      Cells were permeabilized in 0.1% Triton X-100 in PBS for 5 min at room temperature before blocking with blocking buffer (4% goat serum, 1% BSA in PBS-Tween [0.05%]) for 30 min at room temperature. Samples were incubated with primary antibody (1/1000) and a fluorescence conjugated anti-mouse IgG was used to detect staining.
    • Immunofluorescence analysis of HeLa cells transfected with pTF3 and pRF, staining HSV1 ICP8 Major DNA binding protein with ab20194.

      Cells were fixed in paraformaldehyde for 10 min and then permeabilized with 0.5% Triton X-100 for 30 min. The cells were blocked with 4% BSA + 0.2% Tween for 30 min before incubation for 1 hour at RT with primary antibody (1/200 diluted in PBS-T). An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/2000) was used as the secondary antibody.

    References for Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)

    This product has been referenced in:
    • Albright BS  et al. The putative herpes simplex virus 1 chaperone protein UL32 modulates disulfide bond formation during infection. J Virol 89:443-53 (2015). WB . Read more (PubMed: 25320327) »
    • Muylaert I  et al. UL52 primase interactions in the herpes simplex virus 1 helicase-primase are affected by antiviral compounds and mutations causing drug resistance. J Biol Chem 289:32583-92 (2014). WB, ICC/IF . Read more (PubMed: 25278021) »

    See all 14 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample Human Cell (U2OS)
    Specification U2OS
    Permeabilization Yes - 0.5% tritonx-100
    Fixative Paraformaldehyde
    Username

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    Verified customer

    Submitted Nov 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"