Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)

Overview

  • Product name
    Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2]
    See all HSV1 ICP8 Major DNA binding protein primary antibodies
  • Description
    Mouse monoclonal [11E2] to HSV1 ICP8 Major DNA binding protein
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with HSV1. Not yet tested in other species.
  • Immunogen

    Full length native protein (purified from U-35-VERO cells) (HSV).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    11E2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
ICC/IF
  • Application notes
    ICC/IF: Use at an assay dependent dilution (PMID 18434395).
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1.
    • Alternative names
      • DBP antibody
      • Herpes simplex virus type 1 DBP antibody
      • HSV1 DBP antibody
      • ICP8 antibody
      • Infected cell protein 8 antibody
      • Major DNA binding protein antibody
      • UL29 antibody
      see all

    Images

    • ab20194 staining HSV1 ICP8 Major DNA binding protein in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunofluorescence analysis of cells infected with HSV1, staining HSV1 ICP8 Major DNA binding protein (purple) with ab20194, 7 (left) or 17 (right) hours after infection.

      Cells were permeabilized in 0.1% Triton X-100 in PBS for 5 min at room temperature before blocking with blocking buffer (4% goat serum, 1% BSA in PBS-Tween [0.05%]) for 30 min at room temperature. Samples were incubated with primary antibody (1/1000) and a fluorescence conjugated anti-mouse IgG was used to detect staining.
    • Immunofluorescence analysis of HeLa cells transfected with pTF3 and pRF, staining HSV1 ICP8 Major DNA binding protein with ab20194.

      Cells were fixed in paraformaldehyde for 10 min and then permeabilized with 0.5% Triton X-100 for 30 min. The cells were blocked with 4% BSA + 0.2% Tween for 30 min before incubation for 1 hour at RT with primary antibody (1/200 diluted in PBS-T). An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/2000) was used as the secondary antibody.

    References

    This product has been referenced in:
    • Dembowski JA  et al. Replication-Coupled Recruitment of Viral and Cellular Factors to Herpes Simplex Virus Type 1 Replication Forks for the Maintenance and Expression of Viral Genomes. PLoS Pathog 13:e1006166 (2017). Read more (PubMed: 28095497) »
    • Millet R  et al. Impact of the MRN Complex on Adeno-Associated Virus Integration and Replication during Coinfection with Herpes Simplex Virus 1. J Virol 89:6824-34 (2015). Read more (PubMed: 25903339) »

    See all 20 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cell (U2OS)
    Specification
    U2OS
    Permeabilization
    Yes - 0.5% tritonx-100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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