HT1080 whole cell lysate (ab95366)

Overview

  • Product nameHT1080 whole cell lysate
  • General notesDescription: Control cell lysates from HT1080 cells, human connective tissue cells from fibrosarcoma, were cultured in the presence of media containing 10% FCS. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). 200 µg of cellular protein was mixed with the same volume of 2 X SDS-PAGE loading buffer.

    Growth Properties: adherent Morphology: epithelial Tissue: connective tissue Disease: fibrosarcoma Isolation: July, 1972 Virus susceptibility: Human poliovirus 1, RD-114 Feline, Feline leukemia virus, Vesicular stomatitis virus Tumorigenic: Yes Oncogene: ras +


    Cell line isolated from a 35 year old caucasian male. The cells contain an activated N-ras oncogene.

Properties

  • FormLyophilised:Add 100 µl distilled water to the sample. Final concentration is 2 mg/ml.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 5% Glycerol, 1% SDS, 0.002% Bromophenol blue, 31mM Tris HCl, pH 6.8
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notesCell line isolated from a 35 year old caucasian male. The cells contain an activated N-ras oncogene.

    References for HT1080 whole cell lysate (ab95366)

    ab95366 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"