HT29 whole cell lysate (ab3952)

Overview

  • Product nameHT29 whole cell lysate
  • General notesCell line: HT-29 (Human colorectal adenocarcinoma grade II).
    Growth media: McCoy’s 5a medium with 1.5 mM L-glutamine, and 10% FBS.


    HT 29 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applicationsSuitable for: WBmore details

Properties

  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notesHT 29 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas
  • BackgroundHT29 cells are human intestinal epithelial cells which produce the secretory component of Immunoglobulin A (IgA), and carcinoembryonic antigen (CEA). Cells are used for tumourigenicity studies.

Applications

Our Abpromise guarantee covers the use of ab3952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

HT 29 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.

References for HT29 whole cell lysate (ab3952)

ab3952 has not yet been referenced specifically in any publications.

Product Wall

Ich habe folgende Informationen erhalten, die für die Lysate ab3952, ab7900 und ab7909 gelten:

"The cell lines were procured from a reputable source (ATCC) and the flasks and cryopreservation vials are carefully labeled to maintain s...

Read More

Thank you for your call last week and for letting us know about the trouble with ab3952. My colleague is out of the office, but I am following-up with some of her emails. It is my understanding that you would like a credit note for a vial of ab3952 on ...

Read More

Thank you for your enquiry. HT-29 cells are human colonic Adenocarcinoma cells. We offer various loading control antibodies that target house keeping genes for use by various applications. I would recommend that you navigate to www.abcam.com and ho...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"