Overview

  • Product name
  • Description
    Rabbit polyclonal to HTF9C
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 462-621 of Human HTF9C (Q8IZ69).

  • Positive control
    • RT4, EFO-21 and A431 cell lysates. Human oral mucosa tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200.
WB 1/250 - 1/500. Predicted molecular weight: 69 kDa.

Target

  • Function
    May be involved in nucleic acid metabolism and/or modifications.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • HpaII tiny fragments locus 9c protein antibody
    • HTF9C antibody
    • TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
    • TRM2 tRNA methyltransferase 2 homolog A antibody
    • TRM2A_HUMAN antibody
    • TRMT2A antibody
    • tRNA (uracil-5-)-methyltransferase homolog A antibody
    • tRNA methyltransferase 2 homolog A antibody
    • tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121126 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-HTF9C antibody (ab121126) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : EFO-21 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique

    Predicted band size : 69 kDa
    10-20% SDS-PAGE.

References

ab121126 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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