Anti-HTLV I gp46 antibody [67/5.5.13.1] (ab9082)

Overview

  • Product nameAnti-HTLV I gp46 antibody [67/5.5.13.1]
    See all HTLV I gp46 primary antibodies
  • Description
    Mouse monoclonal [67/5.5.13.1] to HTLV I gp46
  • SpecificityReacts with Human T-Lymphotropic Virus Type I (HTLV I) surface protein, gp46 and its precursor to env in RipA. Cross reactivity with the surface protein of HTLV II (MoT) has been observed.
  • Tested applicationsSuitable for: Radioimmunoprecipitation, WB, ELISA, ICC/IF, Flow Cytmore details
  • Immunogen

    Synthetic peptide corresponding to HTLV I gp46 aa 288-312.

  • EpitopeThe epitope is located between amino acids 210-306 of the viral env gene
  • General notes


    The antibody is used to detect HTLV I surface protein and its precursor in lysates prepared from virons or infected cells. Using immunoblotting and/or radioimmunoprecipitation analysis, the biosynthesis and metabolism of viral surface protein may be studied.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesPurified from serum-free culture supernatant. The antibody is greater than 85% pure as determined using denaturing polyacrylamide gel electrophoresis.
  • Primary antibody notesThe antibody is used to detect HTLV I surface protein and its precursor in lysates prepared from virons or infected cells. Using immunoblotting and/or radioimmunoprecipitation analysis, the biosynthesis and metabolism of viral surface protein may be studied.
  • ClonalityMonoclonal
  • Clone number67/5.5.13.1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Radioimmunoprecipitation Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceHTLV I gp46 surface protein is processed from its precursor gp62. It attaches the virus to the host cell by binding to its receptor. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. Fusion occurs at the host cell plasma membrane.
  • Cellular localizationVirion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein
  • Alternative names
    • Glycoprotein 46 antibody
    • SU antibody
    • Surface protein antibody

Anti-HTLV I gp46 antibody [67/5.5.13.1] images

  • Flow Cytometry analysis of human MT2 cells (HTLV-1 transformed T-cell line) using ab9082 (1/250). For further information please refer to abreview.

    See Abreview

References for Anti-HTLV I gp46 antibody [67/5.5.13.1] (ab9082)

This product has been referenced in:
  • Hieshima K  et al. Tax-Inducible Production of CC Chemokine Ligand 22 by Human T Cell Leukemia Virus Type 1 (HTLV-1)-Infected T Cells Promotes Preferential Transmission of HTLV-1 to CCR4-Expressing CD4+ T Cells. J Immunol 180:931-9 (2008). Flow Cyt ; Human . Read more (PubMed: 18178833) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam regarding ab9082. This antibody reacts against a viral glycoprotein.  The glycosylation is variable during the protein production.  Therefore, the western blot is expected to yield a smear or diffuse band near the exp...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (MT2 (HTLV-1 transformed T-cell line))
Specification MT2 (HTLV-1 transformed T-cell line)
Preparation Cell harvesting/tissue preparation method: From cell culture
Sample buffer: PBS
Fixation No fixation. Cells were analyzed just after sample preparation.
Permeabilization No
Gating Strategy No gating. More than 95% of the cells were living cells by trypan blue staining.
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Submitted Jul 28 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"