Overview

  • Product nameAnti-HTLV I p24 antibody [46/3.24.4]
  • Description
    Mouse monoclonal [46/3.24.4] to HTLV I p24
  • SpecificityReacts with Human T-Lymphotropic Virus Type I (HTLV I) core p24. Cross reactivity with p24 from HTLV II has been observed.
  • Tested applicationsSuitable for: ICC/IF, Radioimmunoprecipitation, WB, ELISAmore details
  • Immunogen

    HTVL viral lysate

  • General notes


    The antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from serum-free culture supernatant.
  • Primary antibody notesThe antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.
  • ClonalityMonoclonal
  • Clone number46/3.24.4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 19008946
Radioimmunoprecipitation Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceHuman T-lymphotropic virus (HTLV) belongs to the family Retroviridae, genus Deltaretrovirus. It is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma in adults and may also be involved in certain demyelinating diseases. Adult T-lymphotropic virus (ATLV) is a strain of this disease that effect primarily adults. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localizationCore protein
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • p24 antibody

Anti-HTLV I p24 antibody [46/3.24.4] images

  • Immunofluorescence analysis of HTLV-1-infected T cells, staining HTLV I p24 with ab9081.

    Cells were fixed with paraformaldehyde before incubating with 10% normal goat serum and 0.05% saponin for 30 minutes. Cells were incubated with primary antibody and a fluoroscein-conjugated anti-mouse IgG was used as the secondary antibody.
  • Immunofluorescence analysis of HeLa cells transiently transfected with pEYFP-N3-HTLV-1 Gag, staining HTLV I p24 with ab9081.

    Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% normal donkey serum for 30 minutes. Samples were incubated with primary antibody (1/150 in blocking solution) for 2 hours at room temperature. An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/250) was used as the secondary antibody.

References for Anti-HTLV I p24 antibody [46/3.24.4] (ab9081)

This product has been referenced in:
  • Martin-Latil S  et al. Transcytosis of HTLV-1 across a tight human epithelial barrier and infection of subepithelial dendritic cells. Blood 120:572-80 (2012). Read more (PubMed: 22589473) »
  • Grigsby IF  et al. Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stochiometry. Retrovirology 7:75 (2010). WB, ICC/IF . Read more (PubMed: 20854688) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"