Overview

  • Product name
    Anti-HTR12 antibody
  • Description
    Rabbit polyclonal to HTR12
  • Tested applications
    Suitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana
  • Immunogen

    Synthetic peptide derived from the N terminal domain of Arabidopsis thaliana HTR12

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB 1/200 - 1/2000. Predicted molecular weight: 20 kDa.

Target

  • Relevance
    HTR12 is a histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation and may serve as an epigenetic mark that propagates centromere identity through replication and cell division.
  • Cellular localization
    Nucleus. Centromere. Kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.
  • Database links
    • Alternative names
      • CENH3 antibody
      • Centromeric histone CENH3 antibody
      • F6F3.17 antibody
      • F6F3_17 antibody
      • Histone H3 like centromeric protein HTR12 antibody
      • HTR 12 antibody
      see all

    References

    This product has been referenced in:
    • Karimi-Ashtiyani R  et al. Point mutation impairs centromeric CENH3 loading and induces haploid plants. Proc Natl Acad Sci U S A 112:11211-6 (2015). WB . Read more (PubMed: 26294252) »
    • Mérai Z  et al. The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proc Natl Acad Sci U S A 111:16166-71 (2014). Read more (PubMed: 25344531) »

    See all 2 Publications for this product

    Customer reviews and Q&As


    Unfortunately the immunogen sequence is considered proprietary (commercially sensitive and confidential) and I can not disclose it at this time. However, I can confirm that the immunogen is a peptide of 20 amino acids located between 1 and 40 amin...

    Read More

    Thank you very much for contacting us with your question.

    Unfortunately the immunogen sequence is considered commercially sensitive and we can not disclose it at this time. I can onlyconfirm that it is taken from the first 100 N-terminal amin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up