Overview

  • Product nameAnti-HtrA 1 + 2 + 3 + 4 antibody
  • Description
    Rabbit polyclonal to HtrA 1 + 2 + 3 + 4
  • SpecificityThis antibody recognises both Isoform 1 and Isoform 2.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cat
  • Immunogen

    Synthetic peptide:

    IVSTAQREGRELGLR

    , corresponding to amino acids 273-287 of Human HtrA3. This immunogen sequence is 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4

  • Positive control
    • Heart lysate. Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.2% Gelatin, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 20154083).
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 43 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Anti-HtrA 1 + 2 + 3 + 4 antibody images

    • All lanes : Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112) at 3 µg/ml

      Lane 1 : Human heart lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Rat heart lysate
    • Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112) at 2 µg/ml + Rat heart lysate

      Observed band size : 43 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 65 kDa,80 kDa. We are unsure as to the identity of these extra bands.
    • ab22112 at a 1/200 dilution staining HtrA3 in human bronchial cell line BEAS-2B by Immunocytochemistry/ Immunofluorescence.

      Cells were treated with 20µM etoposide for 24 hours.

    References for Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112)

    This product has been referenced in:
    • Beleford D  et al. High temperature requirement A3 (HtrA3) promotes etoposide- and cisplatin-induced cytotoxicity in lung cancer cell lines. J Biol Chem 285:12011-27 (2010). ICC/IF ; Human . Read more (PubMed: 20154083) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"