Overview

  • Product name
    Anti-htrA antibody
  • Description
    Rabbit polyclonal to htrA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Synechocystis sp
  • Immunogen

    Synthetic peptide:

    QMPPMPNERVQRGT

    , corresponding to amino acids 159-172 of Synechocystis sp. PCC 6803 htrA

  • Positive control
    • Synechocystis sp. PCC 6803 membrane proteins.

Properties

Applications

Our Abpromise guarantee covers the use of ab65481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).

Target

  • Relevance
    htrA belongs to a serine type Deg peptidase family, widespread among bacteria and eukaryotes. This family includes three related members htrA (DegP), HhoA (DegQ) and HhoB (DegS). htrA is a highly hydrophilic protein and probably resides in a soluble compartment. The functions of htrA in cyanobacteria are not studied yet, however, in E. coli, htrA is a heat stress protein that functions in a homohexameric state in the periplasm.
  • Cellular localization
    Periplasm
  • Alternative names
    • htrA antibody
    • Serine protease antibody
    • Slr1204 antibody

Images

  • Anti-htrA antibody (ab65481) at 1/2000 dilution + Synechocystis sp. PCC 6803
    membrane proteins at 3 µg

    Secondary
    anti-rabbit at 1/30000 dilution

    Developed using the ECL technique.

    Predicted band size: 48 kDa
    Observed band size: 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.

References

ab65481 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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