Overview

  • Product name
  • Description
    Rabbit polyclonal to htrA1
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human htrA1 aa 381-412 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 5654

  • Positive control
    • Human placenta tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Protease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
  • Tissue specificity
    Expressed in a variety of tissues, with strongest expression in placenta.
  • Involvement in disease
    Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
    Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
  • Sequence similarities
    Belongs to the peptidase S1B family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD7 antibody
    • CARASIL antibody
    • High-temperature requirement A serine peptidase 1 antibody
    • HtrA antibody
    • HtrA serine peptidase 1 antibody
    • HTRA1 antibody
    • HTRA1_HUMAN antibody
    • IGFBP5 protease antibody
    • L56 antibody
    • ORF480 antibody
    • Protease serine 11 (IGF binding) antibody
    • protease serine 11 antibody
    • PRSS11 antibody
    • Serine protease 11 antibody
    • Serine protease HTRA1 antibody
    • Serine protease HTRA1 precursor antibody
    see all

Anti-htrA1 antibody images

  • Anti-htrA1 antibody (ab38610) at 1/1000 dilution + Human placenta tissue lysate at 35 µg/ml

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling htrA1 with ab38610. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

References for Anti-htrA1 antibody (ab38610)

This product has been referenced in:
  • Goteri G  et al. High temperature requirement A1, transforming growth factor beta1, phosphoSmad2 and Ki67 in eutopic and ectopic endometrium of women with endometriosis. Eur J Histochem 59:2570 (2015). Read more (PubMed: 26708185) »
  • Lorenzi T  et al. The Novel Role of HtrA1 in Gingivitis, Chronic and Aggressive Periodontitis. PLoS One 9:e96978 (2014). IHC-P ; Human . Read more (PubMed: 24979214) »

See all 5 Publications for this product

Product Wall

Thank you for your inquiry.

The immunogen of ab38610 corresponds to the amino acids 395 - 412 of the human HtrA1. http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120509-093551-0608-659902...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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