Overview

  • Product nameAnti-HtrA2 / Omi antibody [EPR22]
    See all HtrA2 / Omi primary antibodies
  • Description
    Rabbit monoclonal [EPR22] to HtrA2 / Omi
  • Tested applicationsWB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on human HtrA2

  • Positive control
    • Jurkat cell lysate, 293T cell lysate, HeLa cell lysate and Human colon tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab75982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC
Flow Cyt
  • Application notesFlow Cyt: 1/70.
    ICC: 1/250 - 1/500.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    IP: 1/10.
    WB: 1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 49 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
    • Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
    • Involvement in diseaseDefects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
    • Sequence similaritiesBelongs to the peptidase S1B family.
      Contains 1 PDZ (DHR) domain.
    • DomainThe mature N-terminus is involved in the interaction with XIAP.
      The PDZ domain mediates interaction with MXI2.
    • Post-translational
      modifications
      Autoproteolytically activated.
    • Cellular localizationMitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.
    • Information by UniProt
    • Database links
    • Alternative names
      • High temperature requirement protein a2 antibody
      • HTRA 2 antibody
      • HtrA like serine protease antibody
      • HtrA serine peptidase 2 antibody
      • HtrA, E. coli, homolog of, 2 antibody
      • HtrA2 antibody
      • HTRA2_HUMAN antibody
      • mitochondrial antibody
      • Omi stress regulated endoprotease antibody
      • Omi stress-regulated endoprotease antibody
      • PARK 13 antibody
      • PARK13 antibody
      • Protease serine 25 antibody
      • PRSS 25 antibody
      • PRSS25 antibody
      • Serine protease 25 antibody
      • Serine protease HTRA2 antibody
      • Serine protease HTRA2 mitochondrial antibody
      • Serine protease htra2 mitochondrial precursor antibody
      • Serine protease omi antibody
      • Serine proteinase OMI antibody
      see all

    Anti-HtrA2 / Omi antibody [EPR22] images

    • Overlay histogram showing HeLa cells stained with ab75982 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75982, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 49 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of paraffin-embedded human colon using ab75982 at 1/100-1/250 dilution.

    References for Anti-HtrA2 / Omi antibody [EPR22] (ab75982)

    This product has been referenced in:
    • Rami A  et al. Translocation of the serine protease Omi/HtrA2 from mitochondria into the cytosol upon seizure-induced hippocampal injury in the neonatal rat brain. Neurochem Res 35:2199-207 (2010). IHC-P ; Rat . Read more (PubMed: 21132459) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (isolated mitochondria from NB4 cell line)
    Loading amount 30 µg
    Specification isolated mitochondria from NB4 cell line
    Gel Running Conditions Reduced Denaturing (13%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Christian Marx

    Verified customer

    Submitted Feb 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"