Anti-HtrA2 / Omi antibody [1B3] (ab33041)

Overview

  • Product name
    Anti-HtrA2 / Omi antibody [1B3]
    See all HtrA2 / Omi primary antibodies
  • Description
    Mouse monoclonal [1B3] to HtrA2 / Omi
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Recombinant human HtrA2/Omi (amino acids 134-458) purified from E. coli.

  • Positive control
    • Extracts of 293T, MCF7, HT29, HL60, HeLa and mouse brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab33041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/2000. Recommended starting dilution is 1/1000. Predicted molecular weight: 49 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
    • Tissue specificity
      Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
    • Involvement in disease
      Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
    • Sequence similarities
      Belongs to the peptidase S1B family.
      Contains 1 PDZ (DHR) domain.
    • Domain
      The mature N-terminus is involved in the interaction with XIAP.
      The PDZ domain mediates interaction with MXI2.
    • Post-translational
      modifications
      Autoproteolytically activated.
    • Cellular localization
      Mitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.
    • Information by UniProt
    • Database links
    • Alternative names
      • High temperature requirement protein A2 antibody
      • HTRA 2 antibody
      • HtrA like serine protease antibody
      • HtrA serine peptidase 2 antibody
      • HtrA, E. coli, homolog of, 2 antibody
      • HtrA2 antibody
      • HTRA2_HUMAN antibody
      • mitochondrial antibody
      • Omi stress regulated endoprotease antibody
      • Omi stress-regulated endoprotease antibody
      • PARK 13 antibody
      • PARK13 antibody
      • Protease serine 25 antibody
      • PRSS 25 antibody
      • PRSS25 antibody
      • Serine protease 25 antibody
      • Serine protease HTRA2 antibody
      • Serine protease HTRA2 mitochondrial antibody
      • Serine protease htra2 mitochondrial precursor antibody
      • Serine protease omi antibody
      • Serine proteinase OMI antibody
      see all

    References for Anti-HtrA2 / Omi antibody [1B3] (ab33041)

    ab33041 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa Cells)
    Loading amount
    20 µg
    Specification
    HeLa Cells
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 01 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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