Anti-HtrA3 antibody - Catalytic domain (ab65911)

Overview

  • Product name
    Anti-HtrA3 antibody - Catalytic domain
    See all HtrA3 primary antibodies
  • Description
    Rabbit polyclonal to HtrA3 - Catalytic domain
  • Specificity
    ab65911 recognizes both of the isoforms of HtrA3.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the catalytic domain of human HtrA3.

  • Positive control
    • Extracts of SW 620 in Serum Free, or treated with TPA, IL 1-Alpha, or IL 1-Beta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects bands of approximately 48 & 40 kDa (predicted molecular weight: 50 & 38 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probable serine protease.
    • Tissue specificity
      Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.
    • Sequence similarities
      Belongs to the peptidase S1B family.
      Contains 1 IGFBP N-terminal domain.
      Contains 1 Kazal-like domain.
      Contains 1 PDZ (DHR) domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • High temperature requirement factor A3 antibody
      • High-temperature requirement factor A3 antibody
      • HtrA serine peptidase 3 antibody
      • HTRA3 antibody
      • HTRA3_HUMAN antibody
      • Pregnancy related serine protease antibody
      • Pregnancy-related serine protease antibody
      • Probable serine protease HTRA3 antibody
      • PRSP antibody
      • Serine protease HTRA3 antibody
      • Tasp antibody
      see all

    Images

    • All lanes : Anti-HtrA3 antibody - Catalytic domain (ab65911) at 1/1000 dilution

      Lane 1 : SW 620 with SF
      Lane 2 : SW 620 with TPA
      Lane 3 : SW 620 with IL 1-Alpha
      Lane 4 : SW 620 with IL 1-Beta
      Lane 5 : Molecular weight markers


      Predicted band size : 50 , 38 kDa
      Observed band size : 40,48 kDa (why is the actual band size different from the predicted?)

    References

    ab65911 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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