Anti-HtrA3 antibody - Catalytic domain (ab65911)

Overview

  • Product nameAnti-HtrA3 antibody - Catalytic domain
    See all HtrA3 primary antibodies
  • Description
    Rabbit polyclonal to HtrA3 - Catalytic domain
  • Specificityab65911 recognizes both of the isoforms of HtrA3.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the catalytic domain of human HtrA3.

  • Positive control
    • Extracts of SW 620 in Serum Free, or treated with TPA, IL 1-Alpha, or IL 1-Beta.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects bands of approximately 48 & 40 kDa (predicted molecular weight: 50 & 38 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProbable serine protease.
    • Tissue specificityWidely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.
    • Sequence similaritiesBelongs to the peptidase S1B family.
      Contains 1 IGFBP N-terminal domain.
      Contains 1 Kazal-like domain.
      Contains 1 PDZ (DHR) domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • High temperature requirement factor A3 antibody
      • High-temperature requirement factor A3 antibody
      • HtrA serine peptidase 3 antibody
      • HTRA3 antibody
      • HTRA3_HUMAN antibody
      • Pregnancy related serine protease antibody
      • Pregnancy-related serine protease antibody
      • Probable serine protease HTRA3 antibody
      • PRSP antibody
      • Serine protease HTRA3 antibody
      • Tasp antibody
      see all

    Anti-HtrA3 antibody - Catalytic domain images

    • All lanes : Anti-HtrA3 antibody - Catalytic domain (ab65911) at 1/1000 dilution

      Lane 1 : SW 620 with SF
      Lane 2 : SW 620 with TPA
      Lane 3 : SW 620 with IL 1-Alpha
      Lane 4 : SW 620 with IL 1-Beta
      Lane 5 : Molecular weight markers


      Predicted band size : 50 , 38 kDa
      Observed band size : 40,48 kDa (why is the actual band size different from the predicted?)

    References for Anti-HtrA3 antibody - Catalytic domain (ab65911)

    ab65911 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab65911.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"