Overview

  • Product name
    Anti-HuD antibody [16C12AA10]
    See all HuD primary antibodies
  • Description
    Mouse monoclonal [16C12AA10] to HuD
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human HuD aa 26-39 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    SNNRNCPSPMQTGA


    Database link: P26378

  • Positive control
    • ELAVL4 recombinant protein.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab171448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa.
ELISA Use a concentration of 1 µg/ml.

Target

  • Function
    May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
    • ELAV L4 antibody
    • ELAV like 4 antibody
    • ELAV like protein 4 antibody
    • ELAV-like protein 4 antibody
    • ELAV4_HUMAN antibody
    • Elavl4 antibody
    • Embryonic lethal abnormal vision Drosophila homolog of like 4 antibody
    • Hu antigen D antibody
    • Hu-antigen D antibody
    • HuD antibody
    • Paraneoplastic encephalomyelitis antigen HuD antibody
    • PNEM antibody
    see all

Images

  • Anti-HuD antibody [16C12AA10] (ab171448) at 2 µg/ml + Human ELAV4 recombinant protein at 0.10000000149 µg

    Secondary
    Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size : 42 kDa
  • Analysis demonstrating the specificity of ab171448 using the ELISA platform.

    Antibody concentration is at 1 µg/mL, the concentration of recombinant proteins is at 250 ng/mL. The antibody reacts specifically to HuD/ELAV4 protein.

References

ab171448 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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