Human 68kDa Neurofilament protein fragment (ab91704)
- Product nameHuman 68kDa Neurofilament protein fragment
- SourceE. coli
- Amino Acid Sequence
- SequenceKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTS SYLMSTRSFPSYYT SHVQEEQIEVEETIEAAKAEEAKD EPPSEGEAEEEEKDKEEAEEEEAAEEEEAA KEESEEAK EEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
- Amino acids391 to 543
Our Abpromise guarantee covers the use of ab91704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Additional NotesProtein Identity confirmed by Mass Spectrometry (MS/MS)(acquired on initial reference batch)
- Concentration information loading...
Preparation and Storage
- Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
- ReconstitutionReconstitution with 139 µl aqua dest.
- 68 kDa neurofilament protein
- 68kDa neurofilament protein
- Light molecular weight neurofilament protein
- Neurofilament light
- Neurofilament light polypeptide
- Neurofilament light polypeptide 68kDa
- Neurofilament protein, light chain
- Neurofilament subunit NF L
- Neurofilament triplet L protein
- FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
- Involvement in diseaseDefects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
- Sequence similaritiesBelongs to the intermediate filament family.
- DomainThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
Human 68kDa Neurofilament protein fragment images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for Human 68kDa Neurofilament protein fragment (ab91704)
ab91704 has not yet been referenced specifically in any publications.