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Anti-human IgG Fab fragment antibody [4A11] (ab771)

Submit an Abreview Q&A (8)Specific References (2)

Overview

  • Product nameAnti-human IgG Fab fragment antibody [4A11]See all Human IgG Fab fragment primary antibodies ...
  • Description
    Mouse monoclonal [4A11] to human IgG Fab fragment
  • SpecificityThis antibody reacts with Fab fragment of human IgG. Specificity was confirmed by Western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions.
  • Tested applicationsFlow Cyt, ELISA, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purification notesMore than 95% pure (by PAGE). Purified from ascites by DEAE chromatography.
  • Clonality Monoclonal
  • Clone number4A11
  • Myelomaunknown
  • IsotypeIgG2b
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: Use at an assay dependent concentration.
ELISA ELISA: Use at an assay dependent concentration.
ICC/IF ICC/IF: Use at an assay dependent concentration.
WB WB: Use at an assay dependent concentration.

Target

  • RelevanceHuman IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.

References for Anti-human IgG Fab fragment antibody [4A11] (ab771)

This product has been referenced in:
  • Lau YF  et al. An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice. J Virol 84:8639-49 (2010). ELISA . Read more (PubMed: 20538850) »
  • Drexler SK  et al. SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of rheumatoid arthritis. Arthritis Rheum 62:2249-61 (2010). Flow Cyt ; Human . Read more (PubMed: 20506350) »

See all 2 Publications for this product

Publishing research using ab771 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab771 Submit a Question for ab771

Displaying 1 - 8 of 8 results for Abreviews and Q&A

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Has product ab771 ever been tested for blocking exposed IgG fab fragment? Is this antibody specific for IgG fab over fc?

We have not performed the blocking assay, but the specificity of this antibody for Fab part of IgG (over Fc) was confirmed by Western blotting using Fab fragments.

Whole human IgG adsorbed to a substrate and am looking to determine the amount of adsorbed protein that has the Fab region exposed (as opposed to the total amount of IgG adsorbed). I was planning on using immunofluorescence (preferably) or ELISA to do th...

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This product will recognize the Fab fragment of any exposed IgG. However if whole IgG is present and not masked by absorption into the substrate, these too will be recognized. I would recommend performing a co stain with an anti- IgG Fc specific antibody ...

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We ordered this antibody. Can you provide the affinity constant KD of this antibody against Human Fab?

Thank you for ordering the Fab fragment antibody . Unfortunately, the affinity or dissociation constant, Kd, is not determined for this antibody. If you have any other questions, please contact us.

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM High background PRIMARY ANTIBODY What is the recommended dilution to use this antibody to detect human IgG on a western blot? SECONDARY ...

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Thank you for your enquiry. For Western blotting, I would recommend using ab771 at a dilution of 1:1000 and optimizing based upon results. At the bottom of the online datasheet for ab771 there is a list of compatible secondary antibodies which can be us...

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ANTIBODY CODE ab771 PRE-SALES ENQUIRY Does it recognise IgG4 isoform and does it detect the light or heavy chain on the fab?

Thank you for your enquiry. The antibody should recognize all human IgG isoforms. The H or L chain specificity has not been determined. If you need any further assistance please contact us again.

I want to buy IgG Fab fragment (ab771). But I need to know the isoelectric point? What is also the isoelectric point of the human IgG(ab436)? thank you for your answer,

Thank you for your enquiry. The isoelectric point of this antibody has not been determined.

Customer would like to know if the epitopes are known for ab74987 and ab771.

Regarding ab7498, this particular clone is Fc-specific but the epitope has not been more precisely determined. For ab771, the epitope is unknown. If you have any more questions, please contact us again.

Customer would like to know which would be better to detect human IgG - anti-Fab or anti-Fc?

The originator of these two antibodies has informed me that the anti Fab fragment antibody is better (IgG receptors bind IgG via Fc so it may be occupied). The Fc fragment is not good for IgM bound to the B-cell surface, but is great for soluble IgM.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"