Overview

  • Product name
    Human Aconitase 2 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 5 3.1%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 6.9%
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.38 ng/ml
  • Range
    0.46 ng/ml - 30 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 111.1 109.9% - 112.9%
    Cell culture media 104.4 100.1% - 106.5%
    Goat Serum 65.4 64.4% - 66.6%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Aconitase 2 (ACO2) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Aconitase 2 protein in human cell and tissue extract samples.


     


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    ACO2 is the mitochondrial form of aconitase, an enzyme that catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab188396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Three concentrations (within the working range of the assay) of the cell and tissue extracts were analyzed in duplicates with this kit. The concentrations of Aconitase 2 were interpolated from data values using Aconitase 2 standard curve, corrected for sample dilution, and graphed in ng of Aconitase 2 per µg of extract (mean +/- SD, n=3).

  • Cytosolic (C), membrane-containing mitochondrial (M) and nuclear (N) fractions were prepared using Cell Fractionation Kit (ab109719). One volume of 5X Cell Extraction Buffer PTR was added to four volumes of each subcellular fractions as well as to the starting material used for the fractionation - whole cell suspension (W), and the extracts were prepared as described in the Sample Preparation section. The extracts were diluted 50X in the 1X Cell Extraction Buffer PTR and analyzed in duplicates with this kit. The concentrations of Aconitase 2 were interpolated from data values using Aconitase 2 standard curve, corrected for sample dilution, and graphed (mean +/- SD, n=2) in ng of Aconitase 2 per mL of extract. All extracts were derived from 2.64 x 106 cell/mL.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab188396 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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