The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activity<1% APC activity. Assay is performed to determine percent contaminating APC activity. APC activity is based on a APC standard curve. Chromagenic assay uses Spectrozyme-TH as substrate.
This product is the active site-blocked form containing Dansyl-EGR-chloromethlyketone. ab62396 does not have the active site blocked.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Protein C (inactivator of coagulation factors Va and VIIIa)
Vitamin K dependent protein C
FunctionProtein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Tissue specificityPlasma; synthesized in the liver.
Involvement in diseaseThrombophilia due to protein C deficiency, autosomal dominant Thrombophilia due to protein C deficiency, autosomal recessive
Post-translational modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.