Human ADAMTS4 ELISA Kit (ab213753)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 625 pg/ml - 40000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ADAMTS4 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 1.127ng/ml 0.046 = 4.1% 2 16 4.29ng/ml 0.184 = 4.3% 3 16 29.063ng/ml 1.627 = 5.6% Inter-assay Sample n Mean SD CV% 1 24 1.21ng/ml 0.071 = 5.9% 2 24 4.672ng/ml 0.201 = 4.3% 3 24 29.313ng/ml 2.081 = 7.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
625 pg/ml - 40000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human ADAMTS4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213753) is designed for the quantitative measurement of Human ADAMTS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: F213-C685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS4 amount of sample captured in plate.
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Notes
ADAMTS4, a disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human ADAMTS4 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human ADAMTS4 antibody 1 x 100µl Lyophilized recombinant Human ADAMTS4 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site. -
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 4
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 4
see all -
Database links
- Entrez Gene: 9507 Human
- Omim: 603876 Human
- SwissProt: O75173 Human
- Unigene: 211604 Human
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab213753 has been referenced in 2 publications.
- Khanam R et al. Identification of Adamts4 as a novel adult cardiac injury biomarker with therapeutic implications in patients with cardiac injuries. Sci Rep 12:9898 (2022). PubMed: 35701493
- Yang Y et al. LincRNA-SLC20A1 (SLC20A1) promotes extracellular matrix degradation in nucleus pulposus cells in human intervertebral disc degeneration by targeting the miR-31-5p/MMP3 axis. Int J Clin Exp Pathol 12:3632-3643 (2019). PubMed: 31934214